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c1000 touch thermal cycler  (Bio-Rad)


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    Structured Review

    Bio-Rad c1000 touch thermal cycler
    C1000 Touch Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 22420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c1000+touch+thermal+cycler+cfx96+real+time+system/CFX96+Touch+Deep+Well+Real-Time+PCR+Detection+System/pm42833467-85-24-28
    Average 96 stars, based on 22420 article reviews
    c1000 touch thermal cycler - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Gene Expression:

    Article Title: RYR1 -Related Myopathies Involve More than Calcium Dysregulation: Insights from Transcriptomic Profiling
    Article Snippet: .. Gene expression was measured by qRT-PCR using the SsoAdvanced Universal Sybr Green Supermix (1725274; Bio-Rad; Hercules, CA, USA) and the C1000 Touch Thermal Cycler-CFX96 Real-Time SYSTEM (Bio-Rad). .. The list of specific primers (Eurofins Scientific; Luxembourg City, Luxembourg) is provided in .

    Quantitative RT-PCR:

    Article Title: RYR1 -Related Myopathies Involve More than Calcium Dysregulation: Insights from Transcriptomic Profiling
    Article Snippet: .. Gene expression was measured by qRT-PCR using the SsoAdvanced Universal Sybr Green Supermix (1725274; Bio-Rad; Hercules, CA, USA) and the C1000 Touch Thermal Cycler-CFX96 Real-Time SYSTEM (Bio-Rad). .. The list of specific primers (Eurofins Scientific; Luxembourg City, Luxembourg) is provided in .

    Article Title: Limiting viral replication in hematopoietic cells delays Rift Valley fever virus disease progression in C57BL/6 mice
    Article Snippet: Samples for quantitative RT-PCR (qRT-PCR) targeting the RVFV L segment were prepared with the Reliance One-Step Multiplex Supermix (BioRad #12010221). .. Reactions were performed using a C1000 Touch Thermal Cycler/CFX96 Real-Time System (Bio-Rad) with the following conditions: 50°C for 15 min, 95°C for 3 min, and then 40 cycles of 95°C for 15 s and 55°C for 1 min. RVFV L RNA template was generated as previously described and serially diluted to known copies per mL in RNase-free water for use as qRT-PCR standard curve template ( ). ..

    Article Title: Limiting viral replication in hematopoietic cells delays Rift Valley fever virus disease progression in C57BL/6 mice.
    Article Snippet: Samples for quantitative RT-PCR (qRT-PCR) targeting the RVFV L segment were prepared with the Reliance One-Step Multiplex Supermix (BioRad #12010221). .. Reactions were performed using a C1000 Touch Thermal Cycler/CFX96 Real-Time System (Bio-Rad) with the following conditions: 50°C for 15 min, 95°C for 3 min, and then 40 cycles of 95°C for 15 s and 55°C for 1 min. RVFV L RNA template was generated as previously described and serially diluted to known copies per mL in RNase-free water for use as qRT-PCR standard curve template (30). ..

    SYBR Green Assay:

    Article Title: RYR1 -Related Myopathies Involve More than Calcium Dysregulation: Insights from Transcriptomic Profiling
    Article Snippet: .. Gene expression was measured by qRT-PCR using the SsoAdvanced Universal Sybr Green Supermix (1725274; Bio-Rad; Hercules, CA, USA) and the C1000 Touch Thermal Cycler-CFX96 Real-Time SYSTEM (Bio-Rad). .. The list of specific primers (Eurofins Scientific; Luxembourg City, Luxembourg) is provided in .

    Article Title: Dual targeting of tumoral cells and immune microenvironment by blocking the IL-33/IL1RL1 pathway.
    Article Snippet: Quantitative real-time PCR was conducted using SsoAdvanced Universal SYBR Green Supermix kit (Cat.No: 172-5270, Bio-Rad) in a total volume of 20μl on a C1000 Touch Thermal Cycler CFX96 Real-Time system (Bio-Rad): 95 °C for 30 s and 40 cycles of denaturation (95 °C for 15 s) and extension (60 °C for 1min). .. Quantitative real-time PCR was conducted using SsoAdvanced Universal SYBR Green Supermix kit (Cat.No: 172-5270, Bio-Rad) in a total volume of 20μl on a C1000 Touch Thermal Cycler CFX96 Real-Time system (Bio-Rad): 95 °C for 30 s and 40 cycles of denaturation (95 °C for 15 s) and extension (60 °C for 1min). ..

    other:

    Article Title: Unbalanced chromatin binding of Polycomb complexes drives neurodevelopmental disorders
    Article Snippet: Total RNA was isolated from frozen cell pellets using TRIzol reagent (Thermo Fisher Scientific cat# 15596018) according to manufacturer’s instructions and extracted using the RNeasy Mini Kit (Qiagen).

    Article Title: SH3 domains selectively activate the PI3 kinase through non-conventional tertiary contacts.
    Article Snippet: ITC analysis AR TI CL E IN P RE SS Isothermal titration calorimetry (ITC) experiments were conducted using a MicroCal PEAQ-ITC (Malvern Panalytical) and a MicroCal iTC200 (GE Healthcare), following the standard 19-injection method at 25 °C.

    Generated:

    Article Title: Limiting viral replication in hematopoietic cells delays Rift Valley fever virus disease progression in C57BL/6 mice
    Article Snippet: Samples for quantitative RT-PCR (qRT-PCR) targeting the RVFV L segment were prepared with the Reliance One-Step Multiplex Supermix (BioRad #12010221). .. Reactions were performed using a C1000 Touch Thermal Cycler/CFX96 Real-Time System (Bio-Rad) with the following conditions: 50°C for 15 min, 95°C for 3 min, and then 40 cycles of 95°C for 15 s and 55°C for 1 min. RVFV L RNA template was generated as previously described and serially diluted to known copies per mL in RNase-free water for use as qRT-PCR standard curve template ( ). ..

    Article Title: Limiting viral replication in hematopoietic cells delays Rift Valley fever virus disease progression in C57BL/6 mice.
    Article Snippet: Samples for quantitative RT-PCR (qRT-PCR) targeting the RVFV L segment were prepared with the Reliance One-Step Multiplex Supermix (BioRad #12010221). .. Reactions were performed using a C1000 Touch Thermal Cycler/CFX96 Real-Time System (Bio-Rad) with the following conditions: 50°C for 15 min, 95°C for 3 min, and then 40 cycles of 95°C for 15 s and 55°C for 1 min. RVFV L RNA template was generated as previously described and serially diluted to known copies per mL in RNase-free water for use as qRT-PCR standard curve template (30). ..

    Standard Curve:

    Article Title: Limiting viral replication in hematopoietic cells delays Rift Valley fever virus disease progression in C57BL/6 mice
    Article Snippet: Samples for quantitative RT-PCR (qRT-PCR) targeting the RVFV L segment were prepared with the Reliance One-Step Multiplex Supermix (BioRad #12010221). .. Reactions were performed using a C1000 Touch Thermal Cycler/CFX96 Real-Time System (Bio-Rad) with the following conditions: 50°C for 15 min, 95°C for 3 min, and then 40 cycles of 95°C for 15 s and 55°C for 1 min. RVFV L RNA template was generated as previously described and serially diluted to known copies per mL in RNase-free water for use as qRT-PCR standard curve template ( ). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Dual targeting of tumoral cells and immune microenvironment by blocking the IL-33/IL1RL1 pathway.
    Article Snippet: Quantitative real-time PCR was conducted using SsoAdvanced Universal SYBR Green Supermix kit (Cat.No: 172-5270, Bio-Rad) in a total volume of 20μl on a C1000 Touch Thermal Cycler CFX96 Real-Time system (Bio-Rad): 95 °C for 30 s and 40 cycles of denaturation (95 °C for 15 s) and extension (60 °C for 1min). .. Quantitative real-time PCR was conducted using SsoAdvanced Universal SYBR Green Supermix kit (Cat.No: 172-5270, Bio-Rad) in a total volume of 20μl on a C1000 Touch Thermal Cycler CFX96 Real-Time system (Bio-Rad): 95 °C for 30 s and 40 cycles of denaturation (95 °C for 15 s) and extension (60 °C for 1min). ..

    Article Title: Limiting viral replication in hematopoietic cells delays Rift Valley fever virus disease progression in C57BL/6 mice.
    Article Snippet: Generated cDNA was used for qPCR with iTaq Universal SYBR Green Super mix (BioRad #1725121) and PrimePCR assays for IFN-α1, IFN-α2, IFN-β, ISG15, or IFIT1 (BioRad #10025636). .. Quantitative PCR was run on a C1000 Touch Thermal Cycler/CFX96 Real-Time System (Bio-Rad) with the following conditions: 94°C for 2 min, 94°C for 15 s, and then 40 cycles of 55°C for 15 s and 68°C for 15 s. Levels of each IFN or ISG in each sample were normalized to CD45 in the PLN or GAPDH in the liver to calculate expression fold change. ..

    Article Title: Limiting viral replication in hematopoietic cells delays Rift Valley fever virus disease progression in C57BL/6 mice
    Article Snippet: Generated cDNA was used for qPCR with iTaq Universal SYBR Green Supermix (BioRad #1725121) and PrimePCR assays for IFN-α1, IFN-α2, IFN-β, ISG15, or IFIT1 (BioRad #10025636). .. Quantitative PCR was run on a C1000 Touch Thermal Cycler/CFX96 Real-Time System (Bio-Rad) with the following conditions: 94°C for 2 min, 94°C for 15 s, and then 40 cycles of 55°C for 15 s and 68°C for 15 s. Levels of each IFN or ISG in each sample were normalized to CD45 in the PLN or GAPDH in the liver to calculate expression fold change. ..

    Expressing:

    Article Title: Limiting viral replication in hematopoietic cells delays Rift Valley fever virus disease progression in C57BL/6 mice.
    Article Snippet: Generated cDNA was used for qPCR with iTaq Universal SYBR Green Super mix (BioRad #1725121) and PrimePCR assays for IFN-α1, IFN-α2, IFN-β, ISG15, or IFIT1 (BioRad #10025636). .. Quantitative PCR was run on a C1000 Touch Thermal Cycler/CFX96 Real-Time System (Bio-Rad) with the following conditions: 94°C for 2 min, 94°C for 15 s, and then 40 cycles of 55°C for 15 s and 68°C for 15 s. Levels of each IFN or ISG in each sample were normalized to CD45 in the PLN or GAPDH in the liver to calculate expression fold change. ..

    Article Title: Limiting viral replication in hematopoietic cells delays Rift Valley fever virus disease progression in C57BL/6 mice
    Article Snippet: Generated cDNA was used for qPCR with iTaq Universal SYBR Green Supermix (BioRad #1725121) and PrimePCR assays for IFN-α1, IFN-α2, IFN-β, ISG15, or IFIT1 (BioRad #10025636). .. Quantitative PCR was run on a C1000 Touch Thermal Cycler/CFX96 Real-Time System (Bio-Rad) with the following conditions: 94°C for 2 min, 94°C for 15 s, and then 40 cycles of 55°C for 15 s and 68°C for 15 s. Levels of each IFN or ISG in each sample were normalized to CD45 in the PLN or GAPDH in the liver to calculate expression fold change. ..



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